The influence of Al<sup>3+</sup>ion on porcine pepsin activity<i>in vitro</i>

Објеката

Тип
Рад у часопису
Верзија рада
објављена верзија
Језик
енглески
Креатор
Vesna M. Pavelkić, Kristina R. Gopcević, Danijela Z. Krstić, Marija A. Ilić
Извор
Journal of Enzyme Inhibition and Medicinal Chemistry
Издавач
Informa UK Limited
Датум издавања
2008
Сажетак
The in vitro effect of Al3þ ions in the concentration range 1.7·1026M–8.7·1023M on pepsin activity at pH 2, via kinetic parameters and its electrophoretic mobility was evaluated. Kinetic study demonstrated the existence of an activation effect of Al3þ at pH 2 on pepsin molecule. Kinetic analysis with respect to concentrations of haemoglobin showed that Al3þ ions increase the maximal velocity (Vmax) and kcat values rather than apparent affinity for substrate (KS) implying the non-competitive nature of activation which indicated that aluminium was a non-essential activator of partial non-competitive type. The values of the equilibrium constants KS and KmA for dissociation of corresponding complexes were evaluated as 0.904 ^ 0.083 mM and 8.56 ^ 0.51 mM, respectively. Dissociation constant KA, of activator from enzyme-activator complex calculated via kinetic and direct measurement of Al3þ binding data, as well as activation constant A50, the activator concentration that gives a rate equal to half at a saturating concentration of activator, were found to be 8.82 ^ 0.90 mM, 8.39 ^ 0.76 mM, and 8.05 ^ 0.48 mM respectively. Native PAGE electrophoresis shows the decrease in electrophoretic mobility of pepsin and confirms modification of the electric charge and conformational changes of pepsin caused by bound Al3þ on the pepsin molecule. Al3þ induced conformational changes of pepsin were verified by UV-VIS and IR spectra. Moreover, the absence of conformational changes in the haemoglobin molecule in the presence of Al3þ ions confirms that the obtained activation is a consequence of conformational changes caused only in the pepsin molecule.
том
23
Број
6
почетак странице
1002
крај странице
1010
doi
10.1080/14756360701841095
issn
1475-6366
Subject
Пепсин, алуминијум, кинетика, активација, електрофоретска мобилност
Pepsin, aluminium, kinetics, activation, electrophoretic mobility
Шира категорија рада
M20
Ужа категорија рада
М23
Права
Отворени приступ
Лиценца
All rights reserved
Формат
.pdf
Скупови објеката
Марија Илић
Radovi istraživača
Медија
14756360701841095.pdf

Vesna M. Pavelkić, Kristina R. Gopcević, Danijela Z. Krstić, Marija A. Ilić. "The influence of Al3+ion on porcine pepsin activityin vitro" in Journal of Enzyme Inhibition and Medicinal Chemistry, Informa UK Limited (2008). https://doi.org/10.1080/14756360701841095

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